CDS
| Accession Number | TCMCG006C10700 |
| gbkey | CDS |
| Protein Id | XP_022565940.1 |
| Location | complement(join(33183858..33184247,33184337..33184453,33184532..33184650,33184746..33184854,33184935..33184973,33185061..33185139,33185210..33185284,33185377..33185441,33185624..33185679,33185795..33185887,33186313..33186571)) |
| Gene | LOC111210015 |
| GeneID | 111210015 |
| Organism | Brassica napus |
Protein
| Length | 466aa |
| Molecule type | protein |
| Topology | linear |
| Data_file_division | PLN |
| dblink | BioProject:PRJNA293435 |
| db_source | XM_022710219.1 |
| Definition | adenylyltransferase and sulfurtransferase MOCS3 [Brassica napus] |
EGGNOG-MAPPER Annotation
| COG_category | H |
| Description | Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor |
| KEGG_TC | - |
| KEGG_Module | - |
| KEGG_Reaction |
R07459
[VIEW IN KEGG] R07461 [VIEW IN KEGG] |
| KEGG_rclass |
RC00043
[VIEW IN KEGG] |
| BRITE |
ko00000
[VIEW IN KEGG] ko00001 [VIEW IN KEGG] ko01000 [VIEW IN KEGG] ko03016 [VIEW IN KEGG] ko04121 [VIEW IN KEGG] |
| KEGG_ko |
ko:K11996
[VIEW IN KEGG] |
| EC |
2.7.7.80
[VIEW IN KEGG]
[VIEW IN INGREDIENT] 2.8.1.11 [VIEW IN KEGG] [VIEW IN INGREDIENT] |
| KEGG_Pathway |
ko04122
[VIEW IN KEGG] map04122 [VIEW IN KEGG] |
| GOs |
GO:0003674
[VIEW IN EMBL-EBI] GO:0003824 [VIEW IN EMBL-EBI] GO:0005575 [VIEW IN EMBL-EBI] GO:0005622 [VIEW IN EMBL-EBI] GO:0005623 [VIEW IN EMBL-EBI] GO:0005737 [VIEW IN EMBL-EBI] GO:0008265 [VIEW IN EMBL-EBI] GO:0016740 [VIEW IN EMBL-EBI] GO:0016782 [VIEW IN EMBL-EBI] GO:0016783 [VIEW IN EMBL-EBI] GO:0044424 [VIEW IN EMBL-EBI] GO:0044464 [VIEW IN EMBL-EBI] |
Sequence
| CDS: ATGGCGGCGAACGGTGGGGATTCGTCGGAAATCGTCCGTGAACTGAAAGAGCTAAAGCTACAGAAAGCGAAAATCGAGCACCGTATCTCTGCTCTCGAAGCAAAGCTCCATGAGACGGCGGCGGTTGAACGCTGCGATGCCGTGTCTAACGGTTGCTCTGTTCCAACGGAGATTGAACACGGGCTTGAACATGGGATGTCTCCTGATCAGATTTACCGTTACAGTCGCCAACTGTTACTCCCTTCGTTTGGTGTTGAAGGGCAGTCAAATCTCTTGAAGTCCTCTGTACTAGTCATCGGAGCTGGAGGGCTTGGTTCACCTGCTTTATTGTATCTGGCAGCTTGTGGTGTTGGTCGATTGGGTATCATCGATCATGATGTTGTAGAACTCAACAATATGCATAGGCAGGTTATACACACGGAAGCATTCATTGGTCACCCCAAAGTGAAATCTGCTGCTACTGCTTGTCGCTCAATAAACTCGACGATCAAGATTGATGAATATGTGGAAGCTCTTCGCACATCCAATGCTTTGGAAATCCTCAGCCAATATGATATCATAGTAGATGCAACCGACAATCCTCCAAGCCGTTACATGATCAGCGATTGTTGTGTTCTCTTAGGAAAGCCTCTGGTGTCAGGTGCTGCGCTTGGAATGGAGGGGCAGCTTACGGTCTATAATCATAAAGGAGGCCCGTGTTACCGTTGTCTATTTTCAACTCCTCCACCGACAACAGCGTGCCAAAGATGTTCTGATAGTGGAGTTCTTGGAGTAGTTCCGGGTGCTATTGGTTGTCTACAAGCGCTAGAGACTATTAAACTCGCTAGCATGGTGGGCGAACCACTCTCTGAACGAATGCTTCTTTTTGACGCTTTATCTGCAAGGATCCGCATTGTCAAGATCAGAGGCAGGTCAGCGCAGTGCACAGTTTGTGGAGACAATTCATCTTTCAATAAGCAGCAGTTCAAGGATTTTGACTATGAGGAGTTCACTCAGTTTCCTTTATCTGCGGGCCCGTTGAATCTACTTCCTGAAGAATCAAGAATCAGCAGCAAAGAGTTCAAAGAAATCCTTCAAAAGAAGGAAAGACATGTTCTTCTAGATGTTCGACCTTCTCATCACTATAAGATTGTCTCTCTCCCGGATTCACTCAACATCCCCTTTGCAAACTTAGAGGCTCGGTTAAACGAGCTTACTTCAGCTCTGAAAGAAAAGGAAGATGGCCATGTTAACTCAGGGTCTTGTGCAAATCCGAGCCTCTACGTTGTTTGCAGGCGAGGGAATGACTCACAGAGAGCTGTTCAGTATCTTCGTGATTCGGGTTTTAGTTCAGCTAAGGATATAATTGGAGGACTTGAGGCTTGGGCAGCTGAAGTCAACCCCAACTTCCCCAGTTACTAG |
| Protein: MAANGGDSSEIVRELKELKLQKAKIEHRISALEAKLHETAAVERCDAVSNGCSVPTEIEHGLEHGMSPDQIYRYSRQLLLPSFGVEGQSNLLKSSVLVIGAGGLGSPALLYLAACGVGRLGIIDHDVVELNNMHRQVIHTEAFIGHPKVKSAATACRSINSTIKIDEYVEALRTSNALEILSQYDIIVDATDNPPSRYMISDCCVLLGKPLVSGAALGMEGQLTVYNHKGGPCYRCLFSTPPPTTACQRCSDSGVLGVVPGAIGCLQALETIKLASMVGEPLSERMLLFDALSARIRIVKIRGRSAQCTVCGDNSSFNKQQFKDFDYEEFTQFPLSAGPLNLLPEESRISSKEFKEILQKKERHVLLDVRPSHHYKIVSLPDSLNIPFANLEARLNELTSALKEKEDGHVNSGSCANPSLYVVCRRGNDSQRAVQYLRDSGFSSAKDIIGGLEAWAAEVNPNFPSY |